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goat polyclonal antibody against nanog  (R&D Systems)


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    R&D Systems goat polyclonal antibody against nanog
    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), <t>NANOG</t> (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .
    Goat Polyclonal Antibody Against Nanog, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody against nanog/product/R&D Systems
    Average 96 stars, based on 663 article reviews
    goat polyclonal antibody against nanog - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment"

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    Journal: bioRxiv

    doi: 10.1101/2024.04.29.591741

    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .
    Figure Legend Snippet: a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .

    Techniques Used: Immunocytochemistry, Microarray

    a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.
    Figure Legend Snippet: a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.

    Techniques Used: Flow Cytometry, Expressing, Control

    Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .
    Figure Legend Snippet: Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .

    Techniques Used: Expressing, Positive Control



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    goat polyclonal antibody against nanog  (R&D Systems)
    96
    R&D Systems goat polyclonal antibody against nanog
    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), <t>NANOG</t> (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .
    Goat Polyclonal Antibody Against Nanog, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody against nanog/product/R&D Systems
    Average 96 stars, based on 1 article reviews
    goat polyclonal antibody against nanog - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    goat polyclonal antibody against nanog  (Novus Biologicals)
    91
    Novus Biologicals goat polyclonal antibody against nanog
    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), <t>NANOG</t> (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .
    Goat Polyclonal Antibody Against Nanog, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody against nanog/product/Novus Biologicals
    Average 91 stars, based on 1 article reviews
    goat polyclonal antibody against nanog - by Bioz Stars, 2026-03
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Immunocytochemistry, Microarray

    a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Flow Cytometry, Expressing, Control

    Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Expressing, Positive Control